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Sensiq Technologies one step gradient mode
One Step Gradient Mode, supplied by Sensiq Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/one+step+gradient+mode/one+step+gradient+mode/pmc04596278-121-10-9
Average 90 stars, based on 1 article reviews
one step gradient mode - by Bioz Stars, 2026-09
90/100 stars

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Article Title: pH responsive granulocyte colony-stimulating factor variants with implications for treating Alzheimer's disease and other central nervous system disorders
Article Snippet: CFPS reactions were performed using a modified, oxidizing PANOx-SP system ( Jewett and Swartz, 2004 ; Zawada et al. , 2011 ) and run at 50 μl scale in a 1.5 ml microcentrifuge tube containing: 12 mM magnesium glutamate, 10 mM ammonium glutamate, 130 mM potassium glutamate, 1.2 mM adenosine triphosphate, 0.85 mM guanosine triphosphate, 0.85 mM uridine triphosphate, 0.85 mM cytidine triphosphate, 0.034 mg/ml folinic acid, 0.171 mg/ml E.coli tRNA (Roche), 2 mM each of all 20 amino acids, 0.03 M phosphoenolpyruvate (Roche), 0.33 mM nicotinamide adenine dinucleotide, 0.27 mM coenzyme-A, 4 mM oxalic acid, 1 mM putrescine, 1.5 mM spermidine, 57 mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), 1 μl/ml Halt protease inhibitor cocktail (Fisher Scientific), 4 mM oxidized glutathione, 1 mM reduced glutathione, 13.3 μg/ml G-CSF plasmid DNA; 6.3 μM disulfide bond isomerase C (DsbC) (Enzo) and 30% (v/v) S30 extract treated with 25 μM iodoacetamide (mixed and incubated at room temperature for 30 min prior to reaction).

Injection:

Article Title: pH responsive granulocyte colony-stimulating factor variants with implications for treating Alzheimer's disease and other central nervous system disorders
Article Snippet: Binding signals were measured using a SensiQ Pioneer surface plasmon resonance (SPR) instrument (SensiQ Technologies). .. The sensor chip was exposed to G-CSF using a SensiQ One Step gradient mode (25 μl/min flow rate) injection with final G-CSF concentrations of 25 and 50 nM for pH 7.4 binding assays and 100 and 200 nM for pH 5.5 assays. ..

Binding Assay:

Article Title: pH responsive granulocyte colony-stimulating factor variants with implications for treating Alzheimer's disease and other central nervous system disorders
Article Snippet: Binding signals were measured using a SensiQ Pioneer surface plasmon resonance (SPR) instrument (SensiQ Technologies). .. The sensor chip was exposed to G-CSF using a SensiQ One Step gradient mode (25 μl/min flow rate) injection with final G-CSF concentrations of 25 and 50 nM for pH 7.4 binding assays and 100 and 200 nM for pH 5.5 assays. ..



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